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biologic duoflow chromatography system  (Bio-Rad)


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    Structured Review

    Bio-Rad biologic duoflow chromatography system
    Biologic Duoflow Chromatography System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 618 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biologic+duoflow+10+system/BioLogic+DuoFlow+10+System/pm41751790-176-11-15
    Average 96 stars, based on 618 article reviews
    biologic duoflow chromatography system - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Purification:

    Article Title: Scale-Up of the Fermentation Process for the Production and Purification of Serratiopeptidase Using Silkworm Pupae as a Substrate
    Article Snippet: .. The concentrated and diafiltrated supernatant was purified using fast protein liquid chromatography (FPLC) with a UNOsphere Q anion exchange column (Bio-Rad, Hercules, CA, USA) on a Biologic DuoFlow 10 system (Bio-Rad). ..

    Article Title: YBR246W is required for the third step of diphthamide biosynthesis
    Article Snippet: .. Cells were lysed using the EmulsiFlex-C3 cell disruptor (Avestin, Inc., Canada), and purified on BioLogic DuoFlow 10 System (Bio-Rad, Hercules, CA). .. The purification was performed on HisTrap HP column (GE Healthcare, Piscataway, NJ) with a linear gradient from 30 mM imidazole to 500 mM imidazole in 30 min.

    Article Title: Genome editing in mammalian cells using the CRISPR type I-D nuclease
    Article Snippet: .. The proteins were further purified by gel-filtration chromatography on pre-equilibrated Superdex 200 Increase 10/30GL (Cytiva) using a Biologic DuoFlow 10 system (Bio-Rad). ..

    Fast Protein Liquid Chromatography:

    Article Title: Scale-Up of the Fermentation Process for the Production and Purification of Serratiopeptidase Using Silkworm Pupae as a Substrate
    Article Snippet: .. The concentrated and diafiltrated supernatant was purified using fast protein liquid chromatography (FPLC) with a UNOsphere Q anion exchange column (Bio-Rad, Hercules, CA, USA) on a Biologic DuoFlow 10 system (Bio-Rad). ..

    Article Title: TRAIL-conjugated liposomes that bind natural killer cells to induce colorectal cancer cell apoptosis.
    Article Snippet: .. SEC was performed with a SuperdexTM 200 increase 10/300 GL (Cytiva) that was integrated with a BioLogic DuoFlow 10 System (Bio-Rad) fast protein liquid chromatography (FPLC) instrument. ..

    Activity Assay:

    Article Title: Identification of the Genes Coding for Carthamin Synthase, Peroxidase Homologs that Catalyze the Final Enzymatic Step of Red Pigmentation in Safflower (Carthamus tinctorius L.).
    Article Snippet: Step 6: Bio-Scale CHT2-I—The enzyme was subjected to chromatography on a Bio-Scale CHT2-I column (Bio-Rad Laboratories; column volume, 2ml) equilibrated with 0.01M potassium phosphate, pH 7.5. .. After washing the column with the equilibration buffer, the enzyme activity was eluted with a linear gradient (0–0.4M) of NaCl in the equilibration buffer (total gradient volume, 20ml) at the flow rate of 1.0ml min-1 using a BioLogic DuoFlow 10 System equipped with a Model 2110 fraction collector (Bio-Rad Laboratories). ..

    Chromatography:

    Article Title: Genome editing in mammalian cells using the CRISPR type I-D nuclease
    Article Snippet: .. The proteins were further purified by gel-filtration chromatography on pre-equilibrated Superdex 200 Increase 10/30GL (Cytiva) using a Biologic DuoFlow 10 system (Bio-Rad). ..

    other:

    Article Title: Multimeric immunotherapeutic complexes activating natural killer cells towards HIV-1 cure
    Article Snippet: Molecules were loaded on a Nickel His-Trap Excel column (Cytiva) over 48 h on a peristaltic pump at a flow rate of 1 ml/min (GE-Healthcare, VWR).



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